ImmunoChemistry Technologies

263 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 97 - 120 of 263 products

Figure 1. Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

Figure 2. Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active PE - Caspase 3 Detection Kit

$506

Figure 1. Jurkat Cells were stimulated with staurosporine (B) or DMSO (A) for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with anti-active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active FITC - Caspase 3 Detection Kit

$506

Figure 1. U937 cells were stimulated with staurosporine for 5 hours, and then assayed for caspase 3/7 activity following the kit protocol.

ImmunoChemistry Technologies Apo 3/7 HTS Assay Kit

From $310

5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2. The ADCC reaction was further incubated for 2 hours at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay.

ImmunoChemistry Technologies aCella - Tox - an assay designed to measure cell cytoxicity

From $883

Figure 1. Assay principle showing the effect of inhibitors

ImmunoChemistry Technologies Bioluminescent Acetylcholinesterase Assay Kit

From $450

Figure shows the two phosphate moieties of ADP, with no trace of a peak indicating the third phosphate of ATP using P31 NMR.

ImmunoChemistry Technologies Ultra Pure ADP by Cell Technology

From $393

ImmunoChemistry Technologies ABTS 1-Component HRP Microwell Substrate (SUBA)

$44

Figure 1. Balb/c mice were either inoculated with HSV-1 virus, or given a sham treatment. Seven days later, the mice were injected IV with either DyLight® 747-VAD-FMK (kit 9114), NIR-DyLight® 747 Free Dye (kit 9115), or no reagent. Strong caspase activity was seen in the brain of the HSV-1 infected / NIR-DyLight® 747 Tracer-treated mouse. The liver is the route of clearance for DyLight® Tracers. All mice show fluorescence signal in the tail where reagent is likely to pool after IV injection.

ImmunoChemistry Technologies Near Infrared DyLight® 747 Free Dye Control Assay

$302

Figure 1. Apoptotic tissues in murine tumor models were imaged in vivo with 747-VAD-FMK (cat. 9114). Negative control (placebo) mice (left) and experimentally treated mice (right) were injected intravenously with DyLight® 747-VAD-FMK tracer and imaged non-invasively at various time points with the CRi Maestro™ imaging system. Apoptotic tumor tissues fluoresce bright blue in the experimental group subjects, indicating that the experimental treatment had an apoptotic effect.

ImmunoChemistry Technologies Near-Infrared DyLight® 747 Tracer in vivo Active Caspase (VAD) Assay

$438

Figure 1. Brain abscesses were induced in mice following inoculation of live S. aureus. Mice received i.v. injections of DyLight® 690-VAD-FMK tracer or DyLight® 690 Dye Control (cat. 9113) 17 hours post-infection; brain tissue was imaged 1 hour later ex vivo using an IVIS® Spectrum™ (Caliper Life Sciences). Strong caspase activity was seen in brain abscesses using DyLight® 690-VAD-FMK tracer (right); minimal signal was detected in the DyLight® 690 Free Dye Control (left).

ImmunoChemistry Technologies Near Infrared DyLight® 690 Free Dye Control Assay

$302

ImmunoChemistry Technologies Near-Infrared DyLight® 690 Tracer in vivo Active Caspase (VAD) Assay

$438

Figure 1. Excitation and emission spectra. Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 630 nm. The emission spectrum (orange) was generated using an excitation of 540 nm. The probe optimally excites at 550-580 nm and has a peak emission at 590-600 nm.

Figure 2. Mice were given either a 0Gy or 15Gy whole lung dose of gamma irradiation. SR in vivo probe (Cat. 982/983) was injected and allowed to circulate for 18 hours prior to sacrifice. Paraffin sections were prepared, nuclei stained with DAPI (blue), and lungs imaged with a fluorescence microscope. Caspase activity (red) was induced in irradiated mice compared to control animals. Data courtesy of E. Hernady (University of Rochester Medical Center, Rochester, NY).

ImmunoChemistry Technologies Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay

From $205.50

Figure 1. Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.

Figure 2. Staurosporine (cat. 6212) was injected into the forebrain of a female house sparrow to induce apoptosis. Approximately 20 hours later, FAM in vivo probe (cat. 980/981) was intravenously injected into the jugular and allowed to circulate 30 minutes. The bird was sacrificed and frozen brain sections prepared. Neurons with active caspases fluoresce green. This image shows one apoptotic neuron at 100X. Data courtesy of Mr. Chris Thompson at the University of Washington, Seattle.

ImmunoChemistry Technologies Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay

From $205

ImmunoChemistry Technologies Sample Diluent Optimization Pack

$187

ImmunoChemistry Technologies Assay Diluent Optimization Pack

$194.50

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained using Swine Annexin V-Fluorescein Assay (cat. 9142). Annexin V-Fluorescein labeled only 13.1% of control cells (UR + LR = 11.0% + 3.1%, left), whereas staurosporine induced Annexin V-Fluorescein labeling in 96.7% of cells (UR + LR = 32.7% + 64.0%, right). Staurosporine also significantly increased the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:19.

ImmunoChemistry Technologies Swine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Equine Annexin V-Fluorescein Assay (Cat. 9141). Equine Annexin V-Fluorescene labeled only 17.4% of the control cells (UR + LR = 10.3% + 7.1%, left); staurosporine induced Equine Annexin V-Fluorescene labeling in 97.6% of cells (UR + LR = 27.8% + 69.8%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:68.

ImmunoChemistry Technologies Equine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.

ImmunoChemistry Technologies Chicken Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained with Canine Annexin V-Fluorescein Assay (cat. 9139). Annexin V-Fluorescein labeled only 15.6% of the control cells (UR + LR = 9.8% + 5.8%, left); staurosporine induced Annexin V-Fluorescein labeling in 97.3% of cells (UR + LR = 11.5% + 85.8%, right). Staurosporine did not significantly increase the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 224:69-71.

ImmunoChemistry Technologies Canine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, and then stained with Bovine Annexin V-Fluorescein (Cat. 9138). Bovine Annexin V-Fluorescein labeled only 17.6% of control cells (UR + LR = 13.5% + 4.1%, left); staurosporine induced Bovine Annexin V-Fluorescein labeling in 98.8% of cells (UR + LR = 15.3% + 83.5%, right). Staurosporine did not increase the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Hanson, ICT, 224:69-71.

ImmunoChemistry Technologies Bovine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Quantification of 4 populations in each sample

ImmunoChemistry Technologies Cell-mediated Cytotoxicity Assay: Total Cytotoxicity Test

From $373

ImmunoChemistry Technologies Recombinant Mouse CCL3

$194

ImmunoChemistry Technologies Recombinant Human IL-17A

$194

ImmunoChemistry Technologies Recombinant Human GM-CSF

$194

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