Anti-Chapsyn-110/PSD-93 Antibody (N18/30)
Our Anti-Chapsyn-110/PSD-93 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N18/30. It is KO validated, detects human, mouse, rabbit, and rat Chapsyn-110/PSD-93, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblot against crude membranes from whole adult mouse brain (MBM) or rat brain (RBM) and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with N18/30 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-057-n18-30-wb-1_{width}x.jpg?v=1717696905)
Immunolabeling of postnatal 14 mouse CA3 specifically labeling PSD93 (Cat no 75-057, 1:400, green) and Ankyrin G(red). DNA labeled with DAPI. Mice were transcardially perfused with ice-cold 4% paraformaldehyde in PBS, then brain removed and postfixed overnight. The 50uM slices were floating sections. Objective 25x, scale bar = 20um. Images kindly provided by Jorge Ramirez-Franco and Oussama El Far, Aix-Marseille Université and INSERM.
![Immunoblot against crude membranes from whole adult mouse brain (MBM) or rat brain (RBM) and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with N18/30 (left) or N52A/42 (right) TC supe.](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-057-n18-30-wb-1_1024x.jpg?v=1717696905)
Human, Mouse, Rabbit, Rat
ICC, IHC, IP, WB
Mouse
KO Validated
Product Specific References for Applications and Species
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Mouse | Rabbit | Rat
- Immunoprecipitation: Mouse | Rat
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 31038014 | 1:200 | Hu, Z., et al. 2019. Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro. Stem Cells Development, 860-870. |
| 27021700 | not listed | Li, X., et al. 2016. Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons. Stem Cells Development, 803-813. |
| 23143515 | 1:100 | Shin, S.M., et al. 2012. GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling.. Nature Neuroscience, 1655-1666. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33185190 | 1:100 | Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058. |
| 26660156 | 1:100 | Kirk, L.M., et al. 2016. Distribution of the SynDIG4/proline-rich transmembrane protein 1 in rat brain.. Journal of Comparative Neurology, 2266-2280. |
| 21357749 | not listed | Vacher, H., et al. 2011. Cdk-mediated phosphorylation of the Kvβ2 auxiliary subunit regulates Kv1 channel axonal targeting.. The Journal of Biology, 813-24. |
| 18509034 | not listed | Ogawa, Y., et al. 2008. Postsynaptic density-93 clusters Kv1 channels at axon initial segments independently of Caspr2.. Journal of Neuroscience, 5731-9. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38663634 | not listed | Ramirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513. |
| 34393723 | 1:500 | Vila A, et al. 2021. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Frontiers in Cellular Neuroscience, 667046. |
| 21958379 | not listed | Duflocq, A., et al. 2011. Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B.. BMC Biology, 1-19. |
| Immunohistochemistry: Rabbit | ||
| PMID | Dilution | Publication |
| 27558197 | 1:500 | Vila, A., et al. 2017. Membrane-associated guanylate kinase scaffolds organize a horizontal cell synaptic complex restricted to invaginating contacts with photoreceptors.. Journal of Comparative Neurology, 850-867. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 24858010 | 1:200 | Fujikawa, T., et al. 2014. Localization of kainate receptors in inner and outer hair cell synapses.. Hearing Research, 20-32. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 24068818 | not listed | Krüger, J.M., et al. 2014. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517. |
| Immunoprecipitation: Rat | ||
| PMID | Dilution | Publication |
| 20089912 | not listed | Ogawa, Y., et al. 2010. ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. . Journal of Neuroscience, 1038-1048. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 38663634 | not listed | Ramirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513. |
| 33229412 | not listed | Pandian, S., et al. 2020. Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, . |
| 30586380 | not listed | Favaro PD, et al. 2018. An opposing function of paralogs in balancing developmental synapse maturation. Plos Biology, e2006838. |
| 30266908 | not listed | Guiberson, N.G.L., et al. 2018. Mechanism-based rescue of Munc18-1 dysfunction in varied encephalopathies by chemical chaperones. Nature Communications, 3986. |
| 28452394 | not listed | Frank, R.A., et al. 2017. Hierarchical organization and genetically separable subfamilies of PSD 95 postsynaptic supercomplexes.. Journal of Neurochemistry, 504-511. |
| 27117477 | 1:2500 | Frank, R.A., et al. 2016. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nature Communications, 11264. |
| 25505322 | not listed | Fortin, D.A., et al. 2014. Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins.. Journal of Neuroscience, 16698-16712. |
| 24361864 | 1:2000 | Dagley, L.F., et al. 2014. Discovery of novel disease-specific and membrane-associated candidate markers in a mouse model of multiple sclerosis. Molecular and Cellular Proteomics, 679-700. |
| 23946397 | not listed | Bonnet, S.A., et al. 2014. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409. |
| 23201971 | 1:5000 | Ryan, T.J., et al. 2014. Evolution of GluN2A/B cytoplasmic domains diversified vertebrate synaptic plasticity and behavior.. Nature Neuroscience, 25-32. |
| 22815516 | 1:500 | Nguyen, M.V., et al. 2012. MeCP2 is critical for maintaining mature neuronal networks and global brain anatomy during late stages of postnatal brain development and in the mature adult brain.. Journal of Neuroscience, 10021-10034. |
| 22246437 | 1:1000 | Zheng, S., et al. 2012. PSD-95 is post-transcriptionally repressed during early neural development by PTBP1 and PTBP2.. Nature Neuroscience, 381-388. |
| 20554866 | not listed | Delint-Ramirez, I., et al. 2010. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93.. Journal of Neuroscience, 8162-8170. |
| 20547132 | not listed | Sumioka, A., et al. 2010. TARP phosphorylation regulates synaptic AMPA receptors through lipid bilayers.. Neuron, 755-767. |
| 19679819 | not listed | Chaudhury, A., et al. 2009. Role of PSD95 in membrane association and catalytic activity of nNOSalpha in nitrergic varicosities in mice gut.. American Journal of Physiology, G806-G813. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 33185190 | 1:500 | Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058. |
| 26247861 | 0.4ug/ml | Levy, J.M., et al. 2015. Synaptic Consolidation Normalizes AMPAR Quantal Size following MAGUK Loss.. Neuron, 534-548. |
| 24705401 | 1:1000 | Bustos, F.J., et al. 2014. PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.. PLoS One, e94037. |
| 23267080 | not listed | Zhao, J.P., et al. 2014. Eye opening and PSD95 are required for long-term potentiation in developing superior colliculus.. PNAS: USA, 707-712. |
| 23486974 | not listed | Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. Journal of Neuroscience, 5040-5052. |
| 20089912 | not listed | Ogawa, Y., et al. 2010. ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. . Journal of Neuroscience, 1038-1048. |
| 20016075 | not listed | Jeffrey, R.A., et al. 2009. Activity-dependent anchoring of importin alpha at the synapse involves regulated binding to the cytoplasmic tail of the NR1-1a subunit of the NMDA receptor.. Journal of Neuroscience, 15613-15620. |
| 17670980 | not listed | Al-Hallaq, R.A., et al. 2007. NMDA di-heteromeric receptor populations and associated proteins in rat hippocampus.. Journal of Neuroscience, 8334-8343. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 20219992 | Yamagata, M., et al. 2010. Synaptic localization and function of Sidekick recognition molecules require MAGI scaffolding proteins.. Journal of Neuroscience, 3579-3588. | |