Anti-Kv4.2 Potassium Channel Antibody (L28/4)

Name: Anti-Kv4.2 Potassium Channel Antibody
Brand: NeuroMab
SKU: 75-361
Price: 319.0 USD
Availability: InStock

7 Citations

Our Anti-Kv4.2 potassium channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L28/4. It is KO validated, detects human, mouse, and rat Kv4.2 potassium channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot against crude brain membranes from adult rat (RBM) and wild-type (WT) and Kv4.2 knockout (KO) mice probed with L28/4 (left) or N52A/42 (right) TC supe.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebellum [HBM(Cb)] or cerebral cortex [HBM(Cx)] and probed with L28/4 (left) or N52A/42 (right) TC supe.$

Sample images for Spp1 (green) and Kv4.2 (cat. 75-361, 1:200; red) showing specific staining of this RGC subclass with silicone oil-induced Spp1 expression. Image from publication CC-BY-4.0. PMID:37624696

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

KO Validated

SKU: 75-361

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Kv4.2 potassium channel

Target Description

Potassium voltage-gated channel subfamily D member 2 or Kv4.2 potassium channel subunit is encoded by the gene KCND2. Kv4.2 is an alpha subunit of the voltage-gated potassium channel (Shal) family and mediates transmembrane potassium transport in excitable membranes. Kv4.2 is an integral membrane protein that combines to form tetramers. Kv4.2 is detected in the heart and brain. In brain it is found at the cell junction, synapse, postsynaptic cell membrane. Diseases associated with this gene include Cycloplegia and early myoclonic encephalopathy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L28/4

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Kcnd2

Molecular Weight

70 kDa

Antigen

Fusion protein amino acids 471-630 (cytoplasmic C-terminus) of rat Kv4.2 (accession number Q63881) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315874

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500
IHC: 1:100

Conjugate

Unconjugated

Specificity

Does not cross-react with Kv4.3 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Potassium voltage-gated channel subfamily D member 2 (RK5) (Shal1) (Voltage-gated potassium channel subunit Kv4.2)

UniProt Details

UniProt (Human): Q9NZV8
UniProt (Immunogen Species): Q63881

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PLA: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37624696	1:200	Zhao, M., et al. 2023. Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy. Cell reports, 113038.
35952672	1:200	Li, L., et al. 2022. Single-cell transcriptome analysis of regenerating RGCs reveals potent glaucoma neural repair genes. Neuron, 2646-2663.
34131060	1:200	Haddjeri-Hopkins, A., et al. 2021. Refining the identity and role of Kv4 channels in mouse substantia nigra dopaminergic neurons. eNeuro, .
31017573	1:100	Carrillo-Reid, L., et al. 2019. Mutant huntingtin enhances activation of dendritic Kv4 K+ channels in striatal spiny projection neurons. . Elife, e40818.

PLA: Mouse
PMID	Dilution	Publication
31639389	not listed	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. . Journal of Molecular and Cellular Cardiology, 93-106.

Western Blot: Mouse
PMID	Dilution	Publication
31639389	1:100	Kilfoil, P.J., et al. 2019. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. . Journal of Molecular and Cellular Cardiology, 93-106.
24795561	1:1000	Lugo, J.N., et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins.. Frontiers in Molecular Neuroscience, 27.

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Anti-Kv4.2 Potassium Channel Antibody (L28/4)

SKU: 75-361

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Kv4.2 Potassium Channel Antibody

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