Our Ku80 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects hamster, human, mouse, and rat Ku80 and is antigen affinity purified. It is great for use in WB.
Immunoprecipitation of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.
Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
XRCC5
Synthetic peptide corresponding to amino acids from an internal sequence of the human Ku80 protein
Hamster, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
PBS + 0.02% NaN3
WB: 1:500-1:1000
WB Brain: 1:500-1:1000
Reacts with the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
XRCC5, X-ray repair cross-complementing protein 5, ATP-dependent DNA helicase 2 subunit 2, Ku86, CTC box-binding factor 85 kDa subunit, Lupus Ku autoantigen protein p86, Thyroid-lupus autoantigen, TLAA, G22P2
