Monoclonal Antibodies

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Surface immunofluorescence (no detergent permeabilization) of cultured rat hippocampal neuron transfected with HA-tagged Kv2.1 plasmid. Red = K39/25. Scale bar = 20 m. Reproduced from 2000 Lim et al Neuron (PMID 10719893).Adult rat brain membrane immunoblot
Adult rat hippocampus immunohistochemistry.Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with untagged KChIP1, KChIP2, KChIP3, KChIP4 or Kv2.1 plasmid probed with N423/75 (left) or K55/82 (right) TC supe.
Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-R- Alpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School).Immunolabelling showing α1-/α5-containing GABAA receptors demonstrated synaptic localizations in a primary hippocampal neuronal culture. Distributions of α1 and α5 (cat. 75-401, 1:1000) subunits (A,B) were co-stained with the inhibitory presynaptic marker protein vGat (C). (D) The α1 subunit was mostly synaptically localized, opposing vGat, whereas α5 was distributed at both the soma and synapse and often showed co-localization with α1-vGat puncta at the synapse (E,F). CC-BY-4.0. PMID:38201218
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green = N144/14, red = Rabbit anti-Myc, blue = Hoechst nuclear stain.Immunoblot against extracts of COS cells transiently transfected with 6xHis/Myc-tagged Olig2 or untagged Kv2.1 plasmid probed with N144/14 (left), 19E10 (middle) or K89/34 (right) TC supe.
array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/4 (green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford). Adult rat hippocampus immunohistochemistry.
Immunofluorescence staining of adult rat optic nerve with K73/20 (red) and βIV-spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistryAnti-SAP97 Antibody
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS-1 cells transiently transfected with KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5 or Kv2.1 plasmids and probed with pure N26A/23.
Immunoblot against crude membranes from adult rat brain (RBM), mouse brain (MBM) and wild-type (WT) and ATAT1 knockout (KO) mouse brains probed with N347/42 (left) or N52A/42 (right) TC supe. WT and KO mouse brains courtesy of Andrea Aguilar and Max Nachury (Stanford
Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/22 (left) or N52A/42 (right) TC supe.  Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274)..
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against isolated cilia and extracts of COS cells transiently transfected with GFP-tagged DNAIC1 or untagged Kv2.1 plasmid probed with UNC 65.56.18.11 (left) or N52A/42 (right) TC supe.
Transfected cell immunoblot: COS cells transiently transfected with Ataxin-1 and Kv2.1 plasmids and probed with N76/8 TC supe.Adult rat brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/8 (left) or K89/41 (right) TC supe.
Adult rat brain membrane immunoblot.
Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).Adult rat whole brain immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA- tagged Kir6.2 or untagged Kv2.1 plasmid probed with N366/60 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).Adult rat hippocampus immunohistochemistry.

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