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Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.Immunoblot on adult mouse brain membranes from two wild-type (WT1,2) and Kvβ2 knockout (Kvβ2 KO) mice. Brain samples courtesy of Dr. Geoff Murphy, Molecular and Behavioral Neuroscience Institute, University of Michigan.
Adult rat brain membrane immunoblotAdult rat whole brain immunohistochemistry
Immunoblot against membrane fractions from adult rat brain (RBM) and heart (RHM) and Kcnv2-S2 mouse wild-type (+/+) and higher-expressing transgenic (Tg/+) brains probed with N448/88 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Jennifer Kearney (Northwestern).
Hippocampus Immunohistochemistry from adult rats treated with kainic acid.Immunohistochemistry against cortex.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/62 (left) or N23B/49 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.
NeuroMab Anti-VGlut2 Antibody
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Adult rat sciatic nerve immuno-fluorescence staining: paranodes labeled with Caspr (green) surround nodes of Ranvier labeled with Beta-IV spectrin (blue) and N168/6 (red). Images courtesy of Shelly Buffington and Matt Rasband (Baylor College of Medicine).Adult rat brain immunohistochemistry N168/6 staining in striatal efferents in globus pallidus.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Neurexin-1-Alpha, Neurexin-1- Beta or untagged Kv2.1 plasmid and probed with N170A/1 TC supe (left) and rabbit anti-Flag (right).
Array tomography immunofluorescence volume reconstruction of 9 serial LRWhite-embedded 70 nm sections from adult mouse cortex layer 6 with L121/25 (green), rabbit Synapsin (Cell Signaling #5297, red). Image courtesy of Kristina Micheva (Stanford).Array tomography immunofluorescence volume reconstruction of 9 serial LRWhite-embedded 70 nm sections from adult mouse cortex layer 6 with L121/25 (green), DAPI (blue). Image courtesy of Kristina Micheva (Stanford).
Adult rat somatosensory cortex immunohistochemistry.Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with L114/81 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot.Immunoblot on membrane fractions of whole rat (RBM) and mouse (MBM) brain and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged VAPA, VAPB or untagged Kv2.1 plasmid probed with N479/107 (left) or N86/8 (right) TC supe..Representative images of transfected neurons (white arrow) expressing GCaMP or MitoPAGFP (green) and VAP CRISPR-Cas9 sgRNAs (e) and immunostained for VAP (cat. 75-496, 1:100;  white) show reduced VAP immunofluorescence compared to untransfected, Control neurons (white arrowheads), identified using Map2. Similar results were obtained with VAP shRNA KD.  Image from publication CC-BY-4.0. PMID:38177103
Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).Immunohistochemistry of coronal brain sections from WT and Cav3.2 KO mice. Reproduced with permission from Mala Shah (University College London, England, UK) and Nature Neuroscience (2011, Huang et al., PMID 21358644).
Cell-surface-expressed ADAM22 (F, magenta) and cell-surface-bound LGI1 (G, magenta) were live-labelled. After fixation and permeabilization, ADAM22 (total) was stained (cat. 75-083; green), with nuclear DNA (blue, F and upper panel in G). ADAM22 S905F is on the cell surface binds to LGI1 as the wild-type. When ADAM22 C401Y is expressed, no cell-surface-bound LGI1 is detected. White squares are total expression of ADAM22 (F) and LGI1–FLAG (lower panel in G, blue). CC-BY-4.0. PMID:37953841Adult rat brain membrane immunoblot.
Immunofluorescence staining of unpermerabilized COS cells transiently transfected with GFP- tagged GluN3A/NR3a and GluN1/NR1 plasmids with N416/40 (red). Images courtesy of Sonia Marco Martinez and Isabel Perez- Otano (University of Navarra).Immunoblot against crude membranes from neonatal or adult rat brain (RBM) and extracts of COS cells transiently transfected with untagged GluN3A/NR3A or Kv2.1 plasmid probed with N416/40 (left) or K35/40 (right) TC supe.
Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.Adult rat brain membrane immunoblot.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or extracts of COS cells transiently transfected with Myc- tagged LRRTM1, LRRTM2 or untagged Kv2.1 plasmid probed with N209C/35 (left), K89/34 (middle) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.
Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).Adult rat hippocampus immunohistochemistry.
Immunoblot versus rat brain low-speed pellet (LSP), mouse brain membranes (MBM) and wild-type (WT) and LRRK2 KO mouse samples probed with N138/6 (left) and K89/41 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson (Johns Hopkins University).
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.1 or untagged Kv2.1 plasmids and probed with N112B/14 (left) and K89/41 (right) TC supe.

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