Primary Antibodies

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Immunocytochemical labeling of WASH relative to F-actin in chick DRG neurons. The cells were labeled with rabbit polyclonal WASH (C-terminal region) antibody (WP4001), then the antibody was detected using appropriate secondary antibody (Green). On the left, this WASH labeling is compared to F-actin staining (Red). (Image provided by Dr. Gianluca Gallo at Drexel University).Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).
Immunohistochemistry of 10 micron cryostat sections of rat brain fixed with 4% formaldehyde by transcardial perfusion showing specific staining of Notch 3.
Western Blot of 10 ug of normal brain lysate showing specific immunolabeling of MBP.
Western Blot of 10 ug of normal human brain lysate showing specific immunolabeling of CNPase.
 Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133.  Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.  Image from the following publ Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133.  Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.  Image from the following publ
Western Blot of 3 ug of RSV virions (subgroup A-Long strain) showing specific immunolabeling of Respiratory Syncytial Virus.  The antibody detects the following RSV proteins: Glycoprotein (G) ~90 kDa, Fusion (F) protein ~55 kDa, and nucleocapsid (N) protein ~46 kDa.  Overexposure of the blot can result in detection of M2 protein (~22 kDA-not shown).
Western Blot of 3 ug of intact A/California/14/2009 H1N1 virions showing specific immunolabeling of Influenza A H1N1.  This antibody detects: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NP) ~26-27 kDa (M and NP often co-migrate as 1 band).
Western Blot of 10 ug of human brain lysate (lane 1), rat brain lysate (lane 2) and mouse brain lysate (lane 3) showing specific immunolabeling of MOR-1.
Western Blot of 10 ug of human brain lysate (lane 1) and rat brain lysate (lane 2) showing specific immunolabeling of Kappa Opioid Receptor / KOR-1.
Immunocytochemistry of primary rat cortical neurons showing specific staining of mGluR1.Immunohistochemistry of rat brain frozen sections (cingulate cortex) showing specific staining  of mGluR1 in red.  DAPI was used to counterstain cell nuclei (blue).
Western Blot of 10ug of human brain lysate (lane 1), rat brain lysate (lane 2) and mouse brain lysate (lane 3) showing specific immunolabeling of Delta Opioid Receptor.
Western Blot of 20 ug of human hippocampus lysate (lanes 2 and 4) showing specific immunolabeling of Noggin and peptide absorption control in lane 3.
Immunohistochemistry of adult rat brain hippocampus showing specific staining of Nurr1.  Tissue was fixed in paraformaldehyde and cut into 20 micron sections.Western Blot of 20 ug of human hippocampus tissue lysate (lanes 2 and 4) showing specific immunolabeling of Nurr1. Peptide absorption control lane 3.

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